REGULAR MAGNESIUM OXIDE
0ppmNo active oxygen
Most magnesium supplements end at the mineral. Let Loose doesn't.
We start with magnesium oxide — an osmotic mineral that draws water into the colon to soften stool and support regularity — and then put it through our proprietary ozonation process, creating a highly ozonated form of magnesium with measurable active oxygen. The result is a formula designed to support gentle, effective elimination from more than one angle.
Active oxygen content measured by iodometric titration, manufacturer lab testing, 2026.
Tested in the lab. 13× the active
oxygen.
Active oxygen is what makes an “ozonated” magnesium actually ozonated. We submitted Let Loose and the two most recognized alternatives to our manufacturer's laboratory and ran iodometric titration — the standard analytical method for quantifying active oxygen content in a dosed supplement.
Here's what came back:
LEADING COMPETITOR
2,050ppmBaseline ozonated magnesium
LET LOOSE DAILY CAPSULES
28,186ppm13× more than competitor
In plain English: magnesium oxide does the proven osmotic work — drawing water into the colon to soften stool and support regularity. What makes Let Loose different is our proprietary ozonation process, which creates a highly ozonated form of magnesium with 13× more measurable active oxygen than the leading ozonated competitor tested.
What ozonated magnesium oxide
actually is
Ozonation is a proprietary process that incorporates oxygen into our magnesium formula using medical-grade ozone. The result is our unique ozonated magnesium ingredient, developed to support digestive health and regularity. That's what makes Let Loose different.*
What happens when you take a Let Loose capsule:
Water moves in.
Magnesium oxide draws water into the colon to help soften stool and support regular bowel movements.1
Our proprietary
ozonation technology.
Our unique manufacturing process enhances magnesium oxide using medical-grade ozone to create our patented ozonated magnesium ingredient.
Digestive support.
Together, these innovations are designed to support digestive health, regularity, and everyday gut wellness.*2,3
The real superpower isn't the
magnesium. It's our proprietary
ozonation technology.
Most of the constipation category talks about magnesium. We wanted to explore whether our proprietary ozonation process could take it one step further. Here's some of the peer-reviewed research that inspired our technology.
The studies below include laboratory and preclinical research on oxygen- and ozone-based approaches. They are provided as scientific background for our proprietary ozonation technology and are not clinical studies of Let Loose capsules.
Research is exploring how ozone may influence the gut environment.
Ozone is a highly reactive form of oxygen that researchers are studying for its effects on the intestinal environment. In a 2025 peer-reviewed study published in the Journal of Medical Microbiology, ozonated water administration in a preclinical dysbiosis model altered microbial populations and supported markers of intestinal barrier function through the SIRT1–Nrf2/HO-1 signaling pathway.3
Plain English: Researchers are studying how ozone-based approaches may influence the gut environment and microbiome. More research is needed to understand how these findings translate to oral ozonated magnesium.
Research is exploring how ozone may support gut barrier function.
The gut barrier is a single-cell-thick layer held together by “tight junction” proteins. When functioning normally, it helps regulate what passes from your digestive tract into your body. Preclinical research has investigated how ozone-based approaches may interact with pathways involved in intestinal barrier integrity. In one 2025 study, researchers observed preservation of barrier function through activation of the aryl hydrocarbon receptor and modulation of tryptophan metabolism.4 A separate preclinical model found that medical ozone influenced the body's endogenous antioxidant defenses in intestinal tissue.5
Plain English: Researchers are continuing to explore how ozone-based approaches may support the intestinal barrier.
Research suggests ozone can interact with microorganisms.
Ozone's interactions with microorganisms have been well characterized in laboratory research. Studies show that ozone can interact with microbial cell membranes and other cellular structures through oxidative mechanisms.6,7 Researchers are continuing to investigate how these properties may translate to applications involving the digestive environment.
Plain English: Ozone has well-documented interactions with microorganisms in laboratory settings. What that means for oral ozonated magnesium in humans still needs to be studied.
Researchers are exploring ozone's relationship with the body's antioxidant response.
Preclinical and clinical research has investigated how controlled ozone-based therapies may influence the body's endogenous antioxidant pathways, including Nrf2, glutathione, and superoxide dismutase.8 While these findings are interesting, additional research is needed to understand how they may apply to oral dietary supplements.
Plain English: Scientists are investigating how controlled ozone exposure may interact with the body's own antioxidant defense systems.
The takeaway: We believe digestive health is about more than just regularity. That's why we developed our proprietary ozonation technology — pairing the established osmotic benefits of magnesium oxide with a highly ozonated ingredient that delivers 13× more measurable active oxygen than the leading ozonated competitor tested.*
Magnesium oxide beats placebo.
Ours starts there.
We're keeping the existing RCT evidence on this page because it's the strongest human data for the mineral itself. The reframe: this is the baseline — what magnesium oxide does on its own, before we add active oxygen.
In a 2019 randomized, double-blind, placebo-controlled trial published in the Journal of Neurogastroenterology and Motility, magnesium oxide improved overall symptoms in 70.6% of participants with occasional constipation, compared with 25% on placebo.1 A 2020 follow-up RCT confirmed it: magnesium oxide matched the efficacy of senna (a stimulant laxative) at roughly 68% response, with a different, non-stimulant mechanism.9
Now imagine that baseline — plus 28,186 ppm of active oxygen in every dose.
70.6%improved on magnesium oxide
(2019 RCT)
25%improved on placebo
(same trial)
~68%response — matched senna with a
non-stimulant mechanism (2020 RCT)
One capsule. Magnesium +
proprietary ozonation. Non-
habit-forming.
Let Loose pairs an RCT-studied osmotic mineral with our proprietary ozonation technology and 13× more measurable active oxygen than the leading ozonated competitor tested. Magnesium oxide helps draw water into the colon to soften stool and support regularity. Our ozonation process is what makes Let Loose different from ordinary magnesium oxide—and we're continuing to study the additional role this unique chemistry may play in digestive health.*
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. If you are pregnant, nursing, taking medication, or have a medical condition, consult your healthcare provider before use.
References
- Mori S, et al. (2019). A Randomized Double-blind Placebo-controlled Trial on the Effect of Magnesium Oxide in Patients With Chronic Constipation. J Neurogastroenterol Motil.
- Fontes B, et al. (2012). Effect of low-dose gaseous ozone on pathogenic bacteria. BMC Infectious Diseases.
- Ozone water enema activates SIRT1-Nrf2/HO-1 pathway to ameliorate gut dysbiosis (2025). Journal of Medical Microbiology.
- Ozone controls the metabolism of tryptophan protecting against sepsis-induced intestinal damage by activating aryl hydrocarbon receptor (2025). World Journal of Gastroenterology.
- Guven A, et al. (2009). Medical ozone therapy reduces oxidative stress and intestinal damage in an experimental model of necrotizing enterocolitis in neonatal rats. Journal of Pediatric Surgery.
- Cho M, et al. (2011). Assessing the microbial oxidative stress mechanism of ozone treatment through the responses of Escherichia coli mutants. Journal of Applied Microbiology.
- Epelle EI, et al. (2023). Ozone application in the food industry: mechanisms of action on microorganisms. PMC.
- Bocci V, et al. (2010). Effect of ozone/oxygen mixture on systemic oxidative stress and organic damage. Toxicology and Industrial Health.
- Morishita D, et al. (2020). Senna Versus Magnesium Oxide for the Treatment of Chronic Constipation: A Randomized, Placebo-Controlled Trial. American Journal of Gastroenterology.
Digestion is the gateway to nutrition
You don't actually get nourished by what you eat. You get nourished by what you digest.
Modern meals are more complex than the digestive system they're landing in. Processed ingredients, dense proteins, refined oils, engineered foods — all of it asks more of your enzymes than nature ever planned for. Koji Digestive Enzymes help your body break food into the small, usable pieces it actually needs. Meals feel lighter. Digestion runs smoother. Your body gets more out of the food you're already eating.
Most enzyme supplements are designed to sound powerful. Ours is dosed to actually perform.
Walk down the enzyme aisle and most labels look impressive: “18 enzymes!” “22-enzyme complex!” Flip them over. The total enzyme content is often around 85 mg, spread across a dozen or more ingredients in trace amounts. That's enough to list on a label. It's not always enough to do the work of digesting a real meal.
Here's the difference:
| Typical Mass-Market Blend | Let Loose Koji Digestive Enzymes |
|---|---|
| ~85 mg total blend | 355 mg per capsule |
| Dozens of enzymes listed, each in trace amounts | 16 enzymes dosed at activity levels sized for real meals |
| Often listed by weight only | Disclosed in enzyme activity units (HUT, DU, FIP, etc.) |
| Commodity sourcing across multiple suppliers | Traditional Japanese Koji fermentation, 150+ year process |
Why activity units matter:
An enzyme's weight tells you how much material is in the capsule, but that includes inactive components too. Activity units tell you how much digestive work it can actually perform. HUT measures protease, DU and SU amylase, FIP lipase, GALU lactase, and ALU alpha-galactosidase. These are standard units used to measure enzyme function. We list every one on our label.
What happens when you take a Let Loose capsule before a meal
You take it with water a few minutes before eating. The capsule dissolves in your stomach, releasing the Koji enzyme blend into the digestive tract right as food arrives. Each enzyme works on a specific part of the meal.
The three things that happen:
Protein gets a head start in the stomach.
Koji-derived fungal proteases are naturally acid-stable and remain active under gastric conditions. Our formula includes acid-stable proteases that start breaking protein apart before it reaches the small intestine.
The full meal gets covered.
Sixteen enzymes work in parallel — proteases on protein, amylases on starch, lipases on oil, lactase on dairy, cellulases on vegetables, alpha-galactosidase on beans.
The good stuff becomes available.
Better breakdown means nutrients locked inside plant cell walls, protein matrices, and fat droplets are released where your gut can absorb them.
See full enzyme breakdown
| Enzyme | Activity per capsule | What it breaks down |
|---|---|---|
| Protease I | 20,000 HUT | Proteins — meats, dairy, legumes |
| Protease II | 50,000 HUT | Proteins — broad pH range |
| Protease (Acid-Stable) | 200 SAPU | Starts protein breakdown in the stomach |
| Peptidase DPP-IV | 72 DPPIV | Peptides and gluten-derived fragments |
| Peptidase | 4,000 HUT | Shorter protein chains |
| Alpha Amylase | 12,000 DU | Starches — bread, rice, potato |
| Alpha Galactosidase | 300 GALU | Complex sugars in beans, cruciferous veg |
| Beta Glucanase | 50 BGU | Beta-glucans in oats, barley |
| Invertase | 400 SU | Sucrose |
| Lactase | 400 ALU | Lactose |
| Pectinase | 14 ENDO PG | Pectin in fruits and vegetables |
| Cellulase | 2,000 CU | Cellulose — plant cell walls |
| Hemicellulase | 2,000 HCU | Hemicellulose — plant fiber |
| Xylanase | 300 XU | Xylan — grain fiber |
| Lipase I | 100 FIP | Fats — short/medium-chain |
| Lipase II | 1,300 FIP | Fats — broad-chain |
Activity units: HUT = protein-breaking units. DU = starch-breaking units. FIP = fat-breaking units. GALU, SU, ALU, BGU, CU, HCU, XU, ENDO PG, DPPIV, SAPU = specialist units for specific substrates.
What one capsule is sized for (theoretical)
One capsule is formulated to support digestion of roughly 40–60 g of protein, 40–80 g of carbohydrate, and 20–25 g of fat. These estimates are biochemical modeling, not clinical claims; actual digestion varies by person.
Why we make this with a 1,100-year-old fermentation method
Koji (Aspergillus oryzae) has been cultivated in Japan for over a millennium for miso, soy sauce, and sake. A single Koji culture naturally secretes many hydrolytic enzymes in parallel.
Koji is built for broad-spectrum digestion.
A single fermentation produces proteases, amylases, lipases, cellulases, hemicellulases, xylanases, pectinases, peptidases, and esterases.
Plain English: Koji gives you a whole team, not a lineup of solo players.
Koji enzymes survive stomach acid.
In in-vitro models of human digestion, fungal enzymes remain active under gastric conditions. Acid-stable proteases can start breaking down protein in the stomach phase.
Plain English: They start working the moment you swallow.
Koji has 150+ years of modern medical use.
Jokichi Takamine patented Taka-diastase, a Koji-derived digestive enzyme preparation, in 1894. Today, A. oryzae is GRAS-designated by the FDA for specified enzyme preparations.
Plain English: This isn't a trend ingredient.
Koji produces secondary enzymes that isolated blends miss.
Traditional fermentation generates cellulases, hemicellulases, xylanases, pectinases, esterases, and additional peptidases that help break apart complex food structures.
Plain English: Before the main enzymes can do their job, something has to open the food up. Koji does both.
The takeaway: Koji fermentation is the reason this formula performs like an integrated digestive system instead of a list of isolated enzymes on a label.
What the research says about digestive enzyme supplementation
The category has become well-studied over the last decade. Here's what peer-reviewed human trials have shown.
Multi-enzyme supplementation reduces post-meal bloating.
A 2024 randomized, double-blind, placebo-controlled crossover study of 25 adults with daily post-meal bloating reported reductions in abdominal distension of 58% at 30 minutes and 68% at 90 minutes compared with placebo; 80% of participants saw a measurable reduction.
Enzyme supplements improve digestive comfort over time.
A 2023 randomized, double-blind, placebo-controlled trial of 120 adults with digestive symptoms reported improvements in quality of life, sleep quality, and post-meal discomfort over two months. Another RCT reported improvements in bloating, fullness, and post-meal distress.
A 2022 INFOGEST in-vitro study demonstrated that fungal-derived digestive enzymes improve macronutrient hydrolysis under simulated gastric and small intestinal conditions. Our formula is built on the same class of enzymes, at 355 mg per capsule.
One capsule before your two biggest meals. That's the whole ritual.
Take one Let Loose Koji Digestive Enzymes capsule before your two largest meals of the day. For heavier meals, take two. Eat what you want. Just digest it better.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Consult your healthcare provider before use. Discontinue if adverse reactions occur.
References
- Morell P, et al. (2022). Fungal digestive enzymes promote macronutrient hydrolysis in the INFOGEST static in vitro simulation of digestion. Food Chemistry.
- Enzyme Technical Association. Orally Administered Enzyme Food Supplement Safety Overview.
- Yang L, et al. (2022). Phenotypic, Genomic, and Transcriptomic Comparison of Industrial Aspergillus oryzae. Microbiology Spectrum.
- Daba GM, et al. (2021). The ancient koji mold as a modern biotechnological tool. Bioresources and Bioprocessing.
- Hiroi M, Nagao S. (2021). Medical Application of Substances Derived from Non-Pathogenic Fungi. Journal of Fungi.
- Spagnuolo R, et al. (2024). A Multi-Digestive Enzyme and Herbal Dietary Supplement Reduces Bloating in a Single Use in Healthy Adults. Nutrition and Dietary Supplements.
- Graziani C, et al. (2023). Efficacy of digestive enzyme supplementation in functional dyspepsia. Biomedicine & Pharmacotherapy.
- Majeed M, et al. (2018). Evaluation of the Safety and Efficacy of a Multienzyme Complex in Patients with Functional Dyspepsia. Journal of Medicinal Food.
Your gut is home to trillions of microbes and a delicate barrier that lines your intestines.
When this ecosystem is out of balance, digestion may suffer — manifesting as irregularity, bloating, gas, or slow transit.
TrioBiotic supports gut health with a three-pronged approach:
Probiotics
Beneficial live microbes that survive digestion and reach the gut.
Postbiotics
Non-live microbial components that still trigger beneficial responses.
Prebiotics & Resistant Starch
Fibers that selectively feed good microbes and promote beneficial by-products (short-chain fatty acids) that nourish your gut lining and beyond.
Together, these ingredients promote:
- A stronger gut barrier
- A healthier microbiome
- Better digestive regularity and comfort
- Support for metabolic health, immunity, and nutrient absorption
The science-backed ingredients in TrioBiotic
Bacillus coagulans (2 Billion CFU)
A spore-forming probiotic that survives stomach acid and bile, produces digestive enzymes and short-chain fatty acids, and supports digestive comfort and microbiota balance.
Bacillus subtilis (2 Billion CFU)
A spore-forming bacterium supporting a diverse microbiome and producing antimicrobial compounds. It supports intestinal barrier integrity and healthy immune signalling.
Akkermansia muciniphila (Heat-Killed AH-39)
A postbiotic associated with the gut mucous layer and intestinal lining. Early research has investigated its relationship with metabolism and barrier function.
Xylooligosaccharides (XOS)
A prebiotic fiber that selectively increases beneficial bacteria such as Bifidobacteria and supports production of short-chain fatty acids.
Galactooligosaccharides (GOS)
A prebiotic fiber that supports Bifidobacteria and Lactobacilli, daily regularity, and gut microbiome balance.
Green Banana Powder (Resistant Starch)
Resistant starch reaches the colon to fuel beneficial microbes and support production of short-chain fatty acids, especially butyrate.
Summary
TrioBiotic combines two spore-forming probiotics, a heat-killed A. muciniphila postbiotic, two prebiotic fibers, and resistant starch from green banana. Together, the formula is designed to support digestion, regularity, the gut barrier, and a resilient microbiome.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Individual results may vary. Consult your healthcare provider before beginning a new supplement regimen.
Read the Papers
- Depommier C, et al. Supplementation with Akkermansia muciniphila in overweight/obese insulin-resistant volunteers: a randomized, double-blind, placebo-controlled pilot study. Nature Medicine, 2019.